Screening of Additive Formulations Enables Off-Chip Drop Reverse Transcription Quantitative Polymerase Chain Reaction of Single Influenza A Virus Genomes
The miniaturization of polymerase chain response (PCR) utilizing drop-based microfluidics permits for amplification of single nucleic acids in aqueous picoliter-sized drops. Correct information assortment throughout PCR requires that drops stay steady to coalescence throughout thermocycling and drop contents are retained. Following systematic testing of recognized PCR components, we recognized an optimized formulation of 1% w/v
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