Category: Enzymes

A simple and highly efficient method of IFI44L methylation detection for the diagnosis of systemic lupus erythematosus

Systemic lupus erythematosus (SLE) is a fancy heterogenous autoimmune illness that may be difficult to diagnose. We beforehand recognized the IFN-induced protein 44-like (IFI44L) methylation marker for SLE analysis, which may be detected by pyrosequencing. Though the earlier method has excessive sensitivity and specificity, it requires particular tools and excessive value for detection. Right here, we established a

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Screening of Additive Formulations Enables Off-Chip Drop Reverse Transcription Quantitative Polymerase Chain Reaction of Single Influenza A Virus Genomes

The miniaturization of polymerase chain response (PCR) utilizing drop-based microfluidics permits for amplification of single nucleic acids in aqueous picoliter-sized drops. Correct information assortment throughout PCR requires that drops stay steady to coalescence throughout thermocycling and drop contents are retained. Following systematic testing of recognized PCR components, we recognized an optimized formulation of 1% w/v

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Quantitative analysis of herpes simplex virus-1 transcript in suspected viral keratitis corneal buttons and its clinical significance

The analysis of Herpes Simplex virus-1 (HSV-1) transcript by totally different investigative strategies (qPCR, PCR and IHC) in corneal buttons from suspected viral keratitis sufferers and the comparability of outcomes with histopathological findings and medical prognosis. Sixty corneal buttons, 30 suspected viral keratitis, and 30 controls (keratoconus and bullous keratopathy) obtained after main penetrating keratoplasty, had been

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