Quantitative analysis of herpes simplex virus-1 transcript in suspected viral keratitis corneal buttons and its clinical significance
The analysis of Herpes Simplex virus-1 (HSV-1) transcript by totally different investigative strategies (qPCR, PCR and IHC) in corneal buttons from suspected viral keratitis sufferers and the comparability of outcomes with histopathological findings and medical prognosis. Sixty corneal buttons, 30 suspected viral keratitis, and 30 controls (keratoconus and bullous keratopathy) obtained after main penetrating keratoplasty, had been included within the research. All of the corneal buttons had been subjected to reverse transcriptase quantitative PCR (qPCR) for the detection of latency-associated transcript (LAT) gene, typical PCR for polymerase (pol) gene, and immunohistochemistry (IHC) for HSV-1 antigen respectively.
After acquiring baseline preoperative medical information, all of the sufferers had been adopted up for 3 years. The outcomes obtained had been correlated with clinicopathological options and follow-up information. (2/30) and HSV-1 antigen in 30% (9/30) circumstances by typical PCR and IHC respectively. A statistically vital affiliation was discovered between qPCR and DNA PCR (P = 0.04). All of the 30 management corneas had been damaging for HSV-1 LAT gene, DNA and antigen. Detection of HSV-1 LAT transcript by qPCR could also be superior to HSV-1 DNA PCR (typical) and IHC, which has low sensitivity. Nonetheless, the utility of HSV-1 LAT mRNA evaluation as a diagnostic modality by qPCR must be validated on a bigger affected person cohort.
Quantitative detection of human adenovirus from river water by monolithic adsorption filtration and quantitative PCR
Water contaminated with fecally derived viruses, often known as enteric viruses, represents a very excessive danger for human well being. Nonetheless, they haven’t been included in water high quality rules but. The detection of those viruses is commonly dearer and time-consuming in comparison with the evaluation of typical fecal indicator organisms. As well as, most strategies usually are not delicate sufficient to detect small viral hundreds that will already trigger severe well being points if current in water. On this research, we established a workflow for the profitable and direct enrichment of human adenovirus (HAdV) from artificially contaminated river water primarily based on monolithic adsorption filtration (MAF) and quantitative polymerase response (qPCR).
With a transparent deal with effectivity, we used focused artificial DNA fragments as normal for the quantification of HAdV by qPCR, resulting in correct and sturdy outcomes with a qPCR effectivity of 95%, a broad working vary over 6 orders of magnitude and an LOD of 1 GU/μL. We carried out a cascade of spiking experiments, enhancing the complexity of the spiking matrix with every step to progressively consider MAF for the direct focus of HAdV. We discovered that negatively charged MAF utilizing monoliths with hydroxyl teams (MAF-OH) confirmed a greater reproducibility and a considerably quicker turnaround time than skimmed milk flocculation (SMF) when concentrating HAdV35 from artificially contaminated, acidified mineral water.
We then validated positively charged MAF utilizing monoliths with diethyl aminoethyl teams (MAF-DEAE) for the direct focus of HAdV5 with out pre-conditioning of water samples utilizing faucet water as spiking matrix with a much less outlined and managed water chemistry. Lastly, we evaluated MAF-DEAE for the direct focus of HAdV5 from floor water utilizing river water as consultant matrix with an undefined water chemistry. We discovered, that MAF-DEAE achieved reproducible recoveries of HAdV5, independently of the spiked focus stage or pattern quantity. Moreover, we confirmed, that MAF-DEAE drastically diminished the restrict of detection (LOD) of HAdV5 by an element of 115 from 6.0 × 103 GU/mL earlier than to five.2 × 101 GU/mL after MAF-DEAE.
We recognized that recoveries elevated for smaller processing volumes with a peak at 0.5 L of 84.0% and confirmed that restoration effectivity is determined by pattern quantity and matrix sort. The right here introduced workflow primarily based on MAF-DEAE and qPCR affords an easy-to-implement and extremely environment friendly different to current approaches and permits for a quick detection of HAdV in water.
Swift and Dependable “Simple Lab” Strategies for the Delicate Molecular Detection of African Swine Fever Virus
African swine fever (ASF) is a contagious viral hemorrhagic illness of home pigs and wild boars. The illness is notifiable to the World Organisation for Animal Well being (OIE) and is answerable for excessive mortality and severe financial losses. PCR and real-time PCR (qPCR) are the OIE-recommended normal strategies for the direct detection of African swine fever virus (ASFV) DNA. The intention of our work was the simplification and standardization of the molecular diagnostic workflow within the lab. For validation of this “straightforward lab” workflow, totally different pattern supplies from animal trials had been collected and analyzed (EDTA blood, serum, oral swabs, chewing ropes, and tissue samples) to establish the optimum pattern materials for diagnostics in reside animals.
Primarily based on our information, the EDTA blood samples or bloody tissue samples signify the very best specimens for ASFV detection within the early and late phases of an infection. The applying of prefilled ready-to-use reagents for nucleic acid extraction or the usage of a Tissue Lysis Reagent (TLR) delivers easy and dependable options for the discharge of the ASFV nucleic acids. For the qPCR detection of ASFV, totally different printed and business kits had been in contrast. Right here, a lyophilized business package reveals the very best outcomes primarily primarily based on the elevated template enter.
 Fast Eva QPCR SuperMix Kit |
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K5052002 |
Biochain |
200 rxn |
EUR 188 |
 Pro QPCR SuperMix Kit |
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K5053200 |
Biochain |
200 reactions |
EUR 225.6 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit |
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K5053400 |
Biochain |
400 reactions |
EUR 358.8 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 Green qPCR SuperMix |
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20-abx098031 |
Abbexa |
-
EUR 1245.60
-
EUR 526.80
-
EUR 760.80
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 Probe qPCR SuperMix |
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20-abx098040 |
Abbexa |
-
EUR 1245.60
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EUR 526.80
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EUR 760.80
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 Library Quantification qPCR SuperMix |
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20-abx098896 |
Abbexa |
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 Green qPCR SuperMix UDG |
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20-abx098032 |
Abbexa |
-
EUR 1362.00
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EUR 543.60
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EUR 794.40
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 Top Green qPCR SuperMix |
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20-abx098033 |
Abbexa |
-
EUR 994.80
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EUR 260.40
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EUR 1362.00
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EUR 493.20
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 Tip Green qPCR SuperMix |
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20-abx098034 |
Abbexa |
-
EUR 1161.60
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EUR 276.00
-
EUR 1596.00
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EUR 560.40
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 QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054200 |
Biochain |
200 reactions |
EUR 489.6 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054400 |
Biochain |
400 reactions |
EUR 712.8 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054004 |
Biochain |
400 rxn |
EUR 635 |
 Pro QPCR SuperMix Kit - ROX premixed |
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K5056200 |
Biochain |
200 reactions |
EUR 225.6 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Pro QPCR SuperMix Kit - ROX premixed |
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K5056400 |
Biochain |
400 reactions |
EUR 358.8 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
 cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems |
Abbexa |
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- 100 rxns ×20 ul Systems
- 50 rxns × 20 ul Systems
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 Fast QCell-Eva One-Step qRT-PCR SuperMix Kit |
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K5054002 |
Biochain |
200 rxn |
EUR 418 |
 Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058200 |
Biochain |
200 reactions |
EUR 253.2 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Fast Pro QPCR SuperMix Kit - ROX premixed |
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K5058400 |
Biochain |
400 reactions |
EUR 384 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
) Top Green qPCR SuperMix (+Dye II) |
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20-abx098890 |
Abbexa |
-
EUR 994.80
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EUR 260.40
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EUR 1362.00
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EUR 493.20
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) Tip Green qPCR SuperMix (+Dye II) |
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20-abx098891 |
Abbexa |
-
EUR 1161.60
-
EUR 276.00
-
EUR 1596.00
-
EUR 560.40
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) cDNA Synthesis SuperMix for qPCR (GC-rich) |
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abx098024-50rxns20ulSystems |
Abbexa |
50 rxns × 20 ul Systems |
EUR 861.6 |
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 HiScript II Q RT SuperMix for qPCR |
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R222-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
 HiScript II Q Select RT SuperMix for qPCR |
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R232-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 282 |
 PLATEMAX ULTRA CLEAR PERMANENT HEAT SEALING FILM FOR QPCR, 100/500 |
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HS-100-QPCR |
CORNING |
100/pk |
EUR 381.6 |
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Description: Sealing Products; Sealing films - Axygen |
 PLATEMAX ULTRA CLEAR PEELABLE HEAT SEALING FILM FOR QPCR. 100/500 |
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HS-150-QPCR |
CORNING |
100/pk |
EUR 381.6 |
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Description: Sealing Products; Sealing films - Axygen |
) HiScript III RT SuperMix for qPCR (+g DNA wiper) |
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R323-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 351.6 |
 PCR SuperMix |
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20-abx098887 |
Abbexa |
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) HiScript II Q Select RT SuperMix for qPCR (+g DNA wiper) |
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R233-01 |
Vazyme |
100 rxn (20 μl/rxn) |
EUR 292.8 |
 Plus PCR SuperMix |
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20-abx098888 |
Abbexa |
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 Library Amplification SuperMix |
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20-abx098894 |
Abbexa |
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) PCR SuperMix (-dye) |
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20-abx098002 |
Abbexa |
-
EUR 493.20
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EUR 226.80
-
EUR 309.60
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) PCR SuperMix (+dye) |
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20-abx098003 |
Abbexa |
-
EUR 493.20
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EUR 226.80
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EUR 7408.80
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EUR 309.60
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 cDNA Synthesis SuperMix |
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20-abx09801420ulSystems |
Abbexa |
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- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
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 OneScriptcDNA Synthesis SuperMix |
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G451 |
ABM |
25 x 20 ul reactions |
EUR 106.8 |
OneScriptcDNA Synthesis SuperMix |
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G452 |
ABM |
100 x 20 ul reactions |
EUR 169.2 |
 Evo? cDNA Supermix |
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M1168-100 |
Biovision |
each |
EUR 457.2 |
Evo? cDNA Supermix |
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M1168-25 |
Biovision |
each |
EUR 320.4 |
 Novo? cDNA Supermix |
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M1169-100 |
Biovision |
each |
EUR 529.2 |
Novo? cDNA Supermix |
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M1169-25 |
Biovision |
each |
EUR 346.8 |
 electrophoresis power supply |
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EV245 |
Consort |
ea |
EUR 574.8 |
 CyanRed Power Box |
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2305226 |
Atto |
2unit |
EUR 339.6 |
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Description: i ndepent l y use for CyanRed Epi |
) T PCR SuperMix (-dye) |
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20-abx098005 |
Abbexa |
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) T PCR SuperMix (+dye) |
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20-abx098006 |
Abbexa |
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) EasyPfu PCR SuperMix (-dye) |
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20-abx098009 |
Abbexa |
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) FastPfu PCR SuperMix (-dye) |
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20-abx098010 |
Abbexa |
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 OneScriptPlus cDNA Synthesis SuperMix |
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G453 |
ABM |
25 x 20 ul reactions |
EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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G454 |
ABM |
100 x 20 ul reactions |
EUR 202.8 |
 COLLECTION CONTAINER 1L, EVA FILM |
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91-200-01 |
CORNING |
1/pk |
EUR 96 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 COLLECTION CONTAINER 2L, EVA FILM |
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91-200-02 |
CORNING |
1/pk |
EUR 105.6 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 COLLECTION CONTAINER 5L, EVA FILM |
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91-200-05 |
CORNING |
1/pk |
EUR 118.8 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 COLLECTION CONTAINER 10L, EVA FILM |
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91-200-10 |
CORNING |
1/pk |
EUR 121.2 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 COLLECTION CONTAINER 20L, EVA FILM |
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91-200-20 |
CORNING |
1/pk |
EUR 132 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
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91-200-36 |
CORNING |
1/pk |
EUR 103.2 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
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91-200-39 |
CORNING |
1/pk |
EUR 106.8 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 5L, EVA FILM |
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91-200-43 |
CORNING |
1/pk |
EUR 181.2 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 10L, EVA FILM |
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91-200-45 |
CORNING |
1/pk |
EUR 151.2 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 20L, EVA FILM |
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91-200-47 |
CORNING |
1/pk |
EUR 158.4 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 COLLECTION CONTAINER 50L, EVA FILM |
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91-200-48 |
CORNING |
1/pk |
EUR 175.2 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 COLLECTION CONTAINER 100L, EVA FILM |
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91-200-82 |
CORNING |
1/pk |
EUR 334.8 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 COLLECTION CONTAINER 200L, EVA FILM |
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91-200-83 |
CORNING |
1/pk |
EUR 350.4 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
 Alkaline phosphatase Power AP |
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AZ140A |
Bio Basic |
1MG |
EUR 120.55 |
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Alkaline phosphatase Power AP |
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AZ235A |
Bio Basic |
1MG |
EUR 139.34 |
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 Four Source Power Supply |
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EDK621 |
Bio Basic |
1 UNIT |
EUR 821.08 |
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 Frit Kit |
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FRIT-KIT |
Next Advance |
1each |
EUR 148.8 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
 Column Packing Kit |
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PACK-KIT |
Next Advance |
1pack |
EUR 1242 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
 Built in power supply for WSE-1165
Power AC 100-240V |
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WSE-650-659 |
Atto |
1unit |
EUR 871.2 |
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Description: Assembl y power suppl y wi t h WSE-1165 such as bui l t -i n |
 COLLECTION CONTAINER 500 ML, EVA FILM |
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91-200-41 |
CORNING |
1/pk |
EUR 82.8 |
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Description: Flexible Containers; Bioprocess Bags and Parts |
) PCR SuperMix for PAGE (+dye) |
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20-abx098004 |
Abbexa |
-
EUR 493.20
-
EUR 226.80
-
EUR 309.60
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 PCR Mycoplasma Detection Kit |
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M034-Kit |
TOKU-E |
Kit |
EUR 319.2 |
 qPCR Control Template Kit |
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G929 |
ABM |
100 rxns |
EUR 177.6 |
 Lentivirus qPCR Quantification Kit |
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K1471-100 |
Biovision |
100 Rxns |
EUR 357.6 |
 Retrovirus qPCR Quantification Kit |
|
K1472-100 |
Biovision |
100 Rxns |
EUR 381.6 |
 Entrans qPCR Probe Kit |
|
RK21209 |
Abclonal |
40 RXN |
Ask for price |
|
|
The great outcomes of the “straightforward lab” technique could possibly be confirmed by the ASFV detection in subject samples from wild boars collected from the 2020 ASFV outbreak in Germany. Acceptable inside management techniques for extraction and PCR are key options of the “straightforward lab” idea and scale back the chance of false-negative and false-positive outcomes. As well as, the usage of easy-to-handle machines and software program reduces coaching efforts and the misinterpretation of outcomes. The PCR diagnostics primarily based on the “straightforward lab” technique can notice a excessive sensitivity and specificity akin to the usual PCR strategies and ought to be particularly usable for labs with restricted experiences and sources.
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