Category: PCR

A simple and highly efficient method of IFI44L methylation detection for the diagnosis of systemic lupus erythematosus

Systemic lupus erythematosus (SLE) is a fancy heterogenous autoimmune illness that may be difficult to diagnose. We beforehand recognized the IFN-induced protein 44-like (IFI44L) methylation marker for SLE analysis, which may be detected by pyrosequencing. Though the earlier method has excessive sensitivity and specificity, it requires particular tools and excessive value for detection. Right here, we established a

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Targeting Epstein-Barr virus oncoprotein LMP1-mediated high oxidative stress suppresses EBV lytic reactivation and sensitizes tumors to radiation therapy

Pathogens corresponding to herpesviruses, Mycoplasma spp., and frog virus 3-like ranavirus have contributed to morbidity and mortality in lots of species of free-living and zoo-maintained chelonians. Nevertheless, their prevalence is understudied in Blanding’s turtles (Emydoidea blandingii) throughout North America. To evaluate the presence of those pathogens, Blanding’s turtles had been sampled in Lake County, Illinois, in 2017 (N = 213) and

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Quantitative analysis of herpes simplex virus-1 transcript in suspected viral keratitis corneal buttons and its clinical significance

The analysis of Herpes Simplex virus-1 (HSV-1) transcript by totally different investigative strategies (qPCR, PCR and IHC) in corneal buttons from suspected viral keratitis sufferers and the comparability of outcomes with histopathological findings and medical prognosis. Sixty corneal buttons, 30 suspected viral keratitis, and 30 controls (keratoconus and bullous keratopathy) obtained after main penetrating keratoplasty, had been

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